T CELL EFFECTOR AND REGULATORY SUBSETS, DIFFERENTIATING BETWEEN ACTIVE AND LATENT MTB INFECTION

Authors

  • Yana Todorova National Centre of Infectious and Parasitic Diseases
  • Radoslava Emilova National Centre of Infectious and Parasitic Diseases
  • Vladimir Milanov Medical University – Sofia, Hospital for Pulmonary Diseases „St. Sophia“, Sofia, Bulgaria
  • Lilia Eneva II Multiprofile hospital for active treatment, Sofia, Bulgaria
  • Assoc. prof. Elizabeta Bachiyska, MD, PhD National Centre of Infectious and Parasitic Diseases
  • Yuliana Atanasova National Centre of Infectious and Parasitic Diseases
  • Ana Baykova National Centre of Infectious and Parasitic Diseases
  • Prof. Maria Nikolova, MD, DSci National Centre of Infectious and Parasitic Diseases

Keywords:

LTBI, Th1/Th17, CD39 Treg, Th17Treg

Abstract

Last generation IFN gamma – based assays (IGRAs) evaluate bulk CD4 and CD8 T cell responses, and do not discriminate between latent and active Micobacterium tuberculosis (MTB) infection. The identification of biomarkers predicting the clinical course and specific therapy effect in latent MTB infection (LTBI) is a major contemporary challenge.

Using multicolor flow cytometry, we compared the levels of circulating CD8 and CD4 effector subsets, in relation to the levels of phenotypically defined regulatory subsets, in two groups of age- and sex-matched MTB-infected individuals: clinically and microbiologically confirmed ATB (n=15), and QFT+ stable LTBI (n=15).

As compared to LTBI subjects, ATB patients are characterized with decreased proportions of CD4 and CD8 CD45RO+CCR7- effectors (14.6% vs. 24%, and 28% vs. 40%, p <0.05 for both), decreased Th1 (10% vs. 16,5 %) and Th1/Th17 (12,5% vs. 21,5%) effector subsets. These changes are accompanied by a significantly increased share of induced (CD39+) FoxP3+CD4Treg (46% vs. 22.6%, p<0.05). The difference affected mostly the Th17-specific (CD39+CCR6+Treg) subset (10.5% vs 4.8%, p<0.05), which correlated inversely with the level of Th1/Th17 effectors (R= -0.5, p<0.05).

In conclusion, we describe a clear-cut distinction between the effector/ regulatory T subset balance in ATB and LTBI. The combined evaluation of Th17Treg and Th1/Th17 effectors in peripheral blood can be employed for MTB-infection monitoring.

ACKNOWLEDGMENTS: This work was supported by the Bulgarian National Science Fund (Research Grant ДН 13/1; 14.12.2017)

Author Biographies

Yana Todorova , National Centre of Infectious and Parasitic Diseases

NRL of Immunology

Radoslava Emilova, National Centre of Infectious and Parasitic Diseases

NRL of Immunology

Vladimir Milanov , Medical University – Sofia, Hospital for Pulmonary Diseases „St. Sophia“, Sofia, Bulgaria

Department of Pulmonary Diseases

Assoc. prof. Elizabeta Bachiyska, MD, PhD, National Centre of Infectious and Parasitic Diseases

Head of NRL of Tuberculosis 

Yuliana Atanasova, National Centre of Infectious and Parasitic Diseases

NRL of Tuberculosis

Ana Baykova, National Centre of Infectious and Parasitic Diseases

NRL of Tuberculosis

Prof. Maria Nikolova, MD, DSci, National Centre of Infectious and Parasitic Diseases

Head of NRL of Immunology

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Published

2019-12-31

How to Cite

Todorova , Y., Emilova, R., Milanov , V., Eneva, L., Bachiyska, E., Atanasova, Y., Baykova, A., & Nikolova, M. (2019). T CELL EFFECTOR AND REGULATORY SUBSETS, DIFFERENTIATING BETWEEN ACTIVE AND LATENT MTB INFECTION. PROBLEMS of Infectious and Parasitic Diseases, 47(1), 30-38. Retrieved from https://pipd.ncipd.org/index.php/pipd/article/view/47_1_6_T_CELL_EFFECTOR_AND_REGULATORY_SUBSETS%2C_DIFFERENTIATING_B

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