DRUG RESISTANCE MUTATIONS AND TRANSMISSION CLUSTERS OF THE HIV-1 CRF01_AE SUB-EPIDEMIC IN BULGARIA

Authors

Keywords:

HIV-1, CRF01_AE, drug resistance, transmission clusters

Abstract

Background. In Bulgaria the second most predominant HIV-1 strain after subtype B is CRF01_AE.

Material and Methods. 270 HIV-1 polymerase (pol) CRF01_AE sequences collected between 1995–2019 were analyzed with the phylogenetic approach. To identify transmission clusters, we used two different genetic distance thresholds (d), i. e. 1.5% and 0.5%.

Results. Genetic distance d=1.5% defined more distant infections in a huge cluster of 154 sequences composed mostly of people, who inject drugs (PWID), whereas when (d) was reduced to 0.5%, we determined more recent transmissions and the large cluster disintegrated into smaller ones. Different drug resistance mutations (DRM) were detected in patient’s HIV-1 pol sequences and were most common in male heterosexual (HET) single sequences.

Conclusions. Our data showed repeated introduction of CRF01_AE in Bulgaria and rapid spread of the infection among PWID groups. Molecular monitoring of the epidemic among PWID communities could help reduce the spread of HIV-1 infection.

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Published

2022-02-25

How to Cite

Grigorova, L., Dimitrova, R., Partsuneva, A., Gancheva, A., Kostadinova, A., Elenkov, I., Yancheva, N., Stoycheva, M., Doychinova, T., Pekova, L., Kosmidis, M., & Alexiev, I. (2022). DRUG RESISTANCE MUTATIONS AND TRANSMISSION CLUSTERS OF THE HIV-1 CRF01_AE SUB-EPIDEMIC IN BULGARIA. PROBLEMS of Infectious and Parasitic Diseases, 49(3), 12–19. Retrieved from https://pipd.ncipd.org/index.php/pipd/article/view/49-3-2-drug-resistance-mutations

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