DRUG RESISTANCE MUTATIONS AND TRANSMISSION CLUSTERS OF THE HIV-1 CRF01_AE SUB-EPIDEMIC IN BULGARIA

Authors

  • Lyubomira Grigorova NCIPD Author
  • Reneta Dimitrova Author https://orcid.org/0000-0001-7711-6798
  • Aleksandra Partsuneva Author
  • Anna Gancheva Author
  • Asya Kostadinova Author
  • Ivaylo Elenkov Author
  • Nina Yancheva Author
  • Mariyana Stoycheva Author
  • Tsetsa Doychinova Author
  • Liliya Pekova Author
  • Minas Kosmidis Author
  • Ivailo Alexiev Author https://orcid.org/0000-0002-3186-1124

DOI:

https://doi.org/10.58395/pipd.v49i3.68

Keywords:

HIV-1, CRF01_AE, drug resistance, transmission clusters

Abstract

Background. In Bulgaria the second most predominant HIV-1 strain after subtype B is CRF01_AE.

Material and Methods. 270 HIV-1 polymerase (pol) CRF01_AE sequences collected between 1995–2019 were analyzed with the phylogenetic approach. To identify transmission clusters, we used two different genetic distance thresholds (d), i. e. 1.5% and 0.5%.

Results. Genetic distance d=1.5% defined more distant infections in a huge cluster of 154 sequences composed mostly of people, who inject drugs (PWID), whereas when (d) was reduced to 0.5%, we determined more recent transmissions and the large cluster disintegrated into smaller ones. Different drug resistance mutations (DRM) were detected in patient’s HIV-1 pol sequences and were most common in male heterosexual (HET) single sequences.

Conclusions. Our data showed repeated introduction of CRF01_AE in Bulgaria and rapid spread of the infection among PWID groups. Molecular monitoring of the epidemic among PWID communities could help reduce the spread of HIV-1 infection.

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Published

2022-02-25

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How to Cite

(1)
Grigorova, L.; Dimitrova, R.; Partsuneva, A.; Gancheva, A.; Kostadinova, A.; Elenkov, I.; Yancheva, N.; Stoycheva, M.; Doychinova, T.; Pekova, L.; Kosmidis, M.; Alexiev, I. DRUG RESISTANCE MUTATIONS AND TRANSMISSION CLUSTERS OF THE HIV-1 CRF01_AE SUB-EPIDEMIC IN BULGARIA. Probl Infect Parasit Dis 2022, 49 (3), 12-19. https://doi.org/10.58395/pipd.v49i3.68.

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