ANTIMICROBIAL SUSCEPTIBILITY OF S. PNEUMONIAE STRAINS ISOLATED FROM CHILDREN WITH NASOPHARYNGEAL CARRIAGE

Authors

  • Mariya Malcheva National Centre of Infectious and Parasitic Diseases
  • Iva Philipova National Centre of Infectious and Parasitic Diseases
  • Chief Assistant Ivan Simeonovski, PhD National Centre of Infectious and Parasitic Diseases
  • Assoc. Prof. Viktoriya Levterova, PhD National Centre of Infectious and Parasitic Diseases
  • Nadyia Brankova National Centre of Infectious and Parasitic Diseases
  • Prof. Todor Kantardjiev, MD National Centre of Infectious and Parasitic Diseases

Keywords:

S. pneumoniae, resistance, pneumococcal serotypes, carriage

Abstract

Streptococcus pneumoniae colonises the nasopharynx of children and could cause life-threatening diseases. As a result of the implementation of conjugate vaccines worldwide the spread of vaccine serotypes has decreased. In Bulgaria PCV10 was introduced in 2010 followed by changes in the invasive clones carrying resistance genes. The aim of our study is to determine the serotype distribution and resistance patterns of isolates from children carriers after vaccination. A total of 834 children were tested for S. pneumoniae and 21% showed positive culture results. All isolates were genotyped with PCR. We found that 85% of the positive samples are from children attending kindergartens and schools. The most frequent serotypes/serogroups were 6C (20%) and 24B/F (11.5%), followed by 3 (8.6%), 11A/D (8%), 35F (6.9%), 19A (6.3%), 23A (6.3%) and 15A/F (6.3%). The susceptibility to β-lactams was high and there were strains showing intermediate susceptibility to benzylpenicillin. This study found 76 (44%) MDR strains non-susceptible to at least 3 antibiotic classes and the most common resistance pattern was erythromycin-clindamycin-tetracycline.

Author Biographies

Mariya Malcheva, National Centre of Infectious and Parasitic Diseases

National Reference Laboratory Molecular Microbiology

Iva Philipova, National Centre of Infectious and Parasitic Diseases

National Reference Laboratory Sexually Transmitted Diseases

Chief Assistant Ivan Simeonovski, PhD, National Centre of Infectious and Parasitic Diseases

National Reference Laboratory Molecular Microbiology

Assoc. Prof. Viktoriya Levterova, PhD, National Centre of Infectious and Parasitic Diseases

National Reference Laboratory Molecular Microbiology

Nadyia Brankova, National Centre of Infectious and Parasitic Diseases

National Reference Laboratory Molecular Microbiology

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Published

2020-05-12

How to Cite

Malcheva, M., Philipova, I., Simeonovski, I., Levterova, V., Brankova, N., & Kantardjiev, T. (2020). ANTIMICROBIAL SUSCEPTIBILITY OF S. PNEUMONIAE STRAINS ISOLATED FROM CHILDREN WITH NASOPHARYNGEAL CARRIAGE. PROBLEMS of Infectious and Parasitic Diseases, 48(1), 15-20. Retrieved from https://pipd.ncipd.org/index.php/pipd/article/view/28

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