FERROPTOSIS IN CD4+ AND CD8+ T-CELLS IN THE SETTINGS OF HIV INFECTION

Authors

  • Radoslava Emilova National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author
  • Yana Todorova National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author
  • Milena Aleksova National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author
  • Reneta Dimitrova National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author
  • Lyubomira Grigorova National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author
  • Damian Vangelov National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author
  • Ivaylo Alexiev National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author
  • Nina Yancheva Specialized Hospital for Active Treatment of Infectious and Parasitic Diseases, Sofia Author
  • Maria Nikolova National Centre of Infectious and Parasitic Diseases - Sofia, Bulgaria Author

DOI:

https://doi.org/10.58395/pmgvqy76

Keywords:

HIV, ferroptosis, cART

Abstract

Introduction: Elevation of intracellular iron concentration triggers ferroptosis. Understanding the regulation and pathophysiological mechanisms of this process in HIV infection may contribute to antiretroviral therapy (cART) monitoring.

Aim: To perform a correlation analysis of the intracellular labile-bound iron pool (LIP) in CD4+ and CD8+ T cells in association with CD4+, CD8+ T cells absolute count (AC) and CD4/CD8 index in HIV+ individuals on continuous cART with sustained viral suppression.

Material and methods: Peripheral blood samples (Li heparin, n=34) were collected in the course of the routine immune monitoring of HIV+ individuals at four time points during 24 months. Plasma HIV viral load (VL) was determined with the Abbott Real-Time HIV-1 test (sensitivity 40 copies/ml). AC and percentage of CD4+ and CD8+ T cells were determined by direct flow cytometry (Multitest, BD Trucount, FACS Canto II). The intracellular content of LIP in CD4 and CD8 T cells (LIP CD4, LIP CD8) was measured at the beginning of the study, using acetoxymethyl ester and subsequent incubation with a chelator (Deferiprone). LIP was quantified according to the mean fluorescence intensity (MFI) (FACSCanto II, Diva 6.1.2).

Results: In the settings of a higher LIP CD4 , high LIP CD8 correlated with increased CD8AC (Rho=0.70, p<0.05) up to 11 (min. 6, max. 15) months after LIP measurement., and decreased CD4/CD8 ratio correlated inversely with LIP CD8 in all consecutive measurements (Rho= -0.71, p<0.01 for all), Importantly, high LIP CD8 correlated with a lower CD4AC (Rho=-0.65, p<0.05) up to five (min.1, max.8) months after LIP measurement.

Conclusion: The increased concentration of intracellular LIP in CD8 cells in HIV+cART individuals could indicate viral activity in the settings of undetectable HIV VL, directly associated with ongoing cell ferroptosis.

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Published

2024-01-25

Issue

Vol. 51 No. 3 (2023)

Section

Articles

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Copyright (c) 2024 Radoslava Emilova, Yana Todorova, Milena Aleksova, Reneta Dimitrova, Lyubomira Grigorova, Damian Vangelov, Ivaylo Alexiev, Nina Yancheva, Maria Nikolova (Author)

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How to Cite

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Emilova, R.; Todorova, Y.; Aleksova, M.; Dimitrova, R.; Grigorova, L.; Vangelov, D.; Alexiev, I.; Yancheva, N.; Nikolova, M. FERROPTOSIS IN CD4+ AND CD8+ T-CELLS IN THE SETTINGS OF HIV INFECTION. Probl Infect Parasit Dis 2024, 51 (3), 5-10. https://doi.org/10.58395/pmgvqy76.

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